The interplay of low H2O2 doses, lytic cyanophage, and Microcystis aeruginosa: Implications for cyanobacterial bloom control and microcystin production/release.

Low H2O2 doses can suppress cyanobacterial blooms without damaging non-target species but enable undesirable regrowth. Besides, the role of cyanophage in preventing regrowth after low H2O2 exposure remains unclear. Applying phages to cyanobacteria pre-exposed to low H2O2 in early growth stages may improve host removal and reduce microcystin (MC) production/release. Lytic cyanophage MDM-1 with a 172 PFU/cell burst size, 2-day short latent period against MCs-producing Microcystis, shows high H2O2 stability. Low H2O2 (1 to 2.5 mg/L) doses significantly (p < 0.05) inhibited Microcystis aeruginosa growth rate, biofilm and MCs concentration reduction in a dose-dependent manner but regrowth occurred at all concentrations. Phage treatment eliminated cells without H2O2 pretreatment within 3 days and reduced MC production. H2O2-pretreated M. aeruginosa cells altered the phage dynamics, affecting adsorption, latency, production, and cell lysis in response to H2O2-induced oxidative stress. At 1.5 mg H2O2/L pretreatment, cells were eliminated with reduced MC production, like untreated cells. H2O2 pretreatment with 2.0 and 2.5 mg/L resulted in an extension of the phage absorption phase and the latent period. This was accompanied by a reduction in lysis efficacy, attributed to the increased ROS production. At 2.5 mg H2O2/L, 17.10 % of phages remain un-adsorbed, with cell lysis rate dropped from 0.89 d-1 to 0.26 d-1 compared to the untreated control. The highest phage titer (70 %) was obtained with 1.5 mg/H2O2 pretreated cells. This study emphasizes that low-dose H2O2 eliminates Microcystis but severely affects phage lysis and MCs release depending on H2O2-induced ROS levels. It is a crucial consideration when using phages to control cyanobacterial blooms with H2O2-induced stress.

Noggin contributes to brain metastatic colonization of lung cancer cells.

BACKGROUND: Brain metastasis is a common complication among patients with lung cancer, yet the underlying mechanisms remain unclear. In this study, we aimed to investigate the pathogenesis of brain metastasis in lung cancer. METHODS: We established highly colonizing metastatic lung cancer cells, A549-M2, through multiple implantations of A549 human lung cancer cells in the carotid artery of athymic nude mice. RESULTS: Compared to parental cells (M0), M2 cells demonstrated slower growth in culture plates and soft agar, as well as lower motility and higher adhesion, key characteristics of mesenchymal-epithelial transition (MET). Further analysis revealed that M2 cells exhibited decreased expression of epithelial-mesenchymal transition markers, including ZEB1 and Vimentin. M2 cells also demonstrated reduced invasiveness in co-culture systems. RNA sequencing and gene set enrichment analysis confirmed that M2 cells underwent MET. Intriguingly, depletion of Noggin, a BMP antagonist, was observed in M2 cells, and replenishment of Noggin restored suppressed migration and invasion of M2 cells. In addition, Noggin knockdown in control M0 cells promoted cell attachment and suppressed cell migration, suggesting that Noggin reduction during brain colonization causes inhibition of migration and invasion of metastatic lung cancer cells. CONCLUSIONS: Our results suggest that lung cancer cells undergo MET and lose their motility and invasiveness during brain metastatic colonization, which is dependent on Noggin.

miR-199a and miR-199b facilitate diffuse gastric cancer progression by targeting Frizzled-6.

Pathological markers that can monitor the progression of gastric cancer (GC) may facilitate the diagnosis and treatment of patients with diffuse GC (DGC). To identify microRNAs (miRNAs) that can differentiate between early and advanced DGC in the gastric mucosa, miRNA expression profiling was performed using the NanoString nCounter method in human DGC tumors. Ectopic expression of miR-199a and miR-199b (miR-199a/b) in SNU601 human GC cells accelerated the growth rate, viability, and motility of cancer cells and increased the tumor volume and weight in a mouse xenograft model. To study their clinicopathological roles in patients with GC, miR-199a/b levels were measured in human GC tumor samples using in situ hybridization. High miR-199a/b expression level was associated with enhanced lymphovascular invasion, advanced T stage, and lymph-node metastasis. Using the 3'-untranslated region (UTR) luciferase assay, Frizzled-6 (FZD6) was confirmed to be a direct target of miR-199a/b in GC cells. siRNA-mediated depletion of FZD6 enhanced the motility of SNU601 cells, and addback of FZD6 restored cancer cell motility stimulated by miR-199a/b. In conclusion, miR-199a/b promotes DGC progression by targeting FZD6, implying that miR-199a/b can be used as prognostic and diagnostic biomarkers for the disease.

Fabrication of Bi2O3/Bismuth Titanates Modified with Metal-Organic Framework-In2S3/CdIn2S4 Materials for Electrocatalytic H2 Production and Its Photoactivity.

Compositional and structural elucidation of the materials is important to know their properties, chemical stability, and electro-photoactivity. The heterojunction electrocatalyst and photocatalyst activity could open a new window for solving the most urgent environmental and energy problems. Here, for the first time, we have designed and fabricated Bi2O3/bismuth titanates modified with MOF-In2S3/CdIn2S4 materials by a stepwise process. The detailed structural elucidation and formation of mixed composite phases were studied in detail. It has been found that the formed composite was efficiently utilized for the electrocatalytic H2 production reaction and the photocatalytic degradation of tetracycline. XRD patterns for the metal-organic framework-In2S3 showed a main compound of MOF, and it was assigned to a MIL-53 MOF phase, with a monoclinic structure. The addition of CdCl2 onto the MOF-In2S3 phase effectively produced a CdIn2S4 flower platform on the MOF rods. The uniform dispersion of the bismuth titanates in MOF-In2S3/CdIn2S4 materials is detected by mapping of elements obtained by dark-field HAADF-STEM. Finally, the predictions of how to integrate experiments and obtain structural results more effectively and their common development in new heterojunctions for electro-/photocatalytic applications are presented.

A downstream process control tool based on the redox dye resazurin for rapid and accurate measurement of microbial metabolic activity.

Many sophisticated water treatment plants need a reliable, fast, and economical microbial load detection method. We refined a colorimetric assay using the redox dye resazurin to assess viable microorganisms. Here, we have used a mixed bacterial suspension of significant multi-drug-resistant coliform bacteria isolated from hospital wastewater and constructed a resazurin reduction calibration curve which could accurately predict the level of microbial contamination. The number of viable microorganisms was calculated from calibration curve in terms of log colony forming units (CFU) per milliliter. Ultrasonication disinfection of bacterial suspension for a duration of 50 min measured by resazurin assay depicted a reduction of 16.94%, 26.48%, and 37.69% at 410 W, 580 W, and 700 W, respectively. A synergistic effect of the combined methods of ultrasonication and heat disinfection treatments on raw wastewater and secondary wastewater effluent was observed and was also evaluated using both resazurin assay and standard plate count method. For raw wastewater, about 1.8 log reduction was observed for ultrasonication alone and 4 log CFU/mL reduction for thermosonication. In the secondary wastewater effluent, a reduction of 2.9 and 3.2 log CFU/mL was recorded for ultrasonication and thermosonication respectively. Resazurin microbial viability test results were highly comparable with conventional colony plate count for all treatment procedures, suggesting its appropriateness for quick and reliable wastewater sample microbial viability monitoring.

Effect of aging on the formation and growth of colonic epithelial organoids by changes in cell cycle arrest through TGF-ß-Smad3 signaling.

BACKGROUND: This study aimed to investigate how aging alters the homeostasis of the colonic intestinal epithelium and regeneration after tissue injury using organoid models and to identify its underlying molecular mechanism. METHODS: To investigate aging-related changes in the colonic intestinal epithelium, we conducted organoid cultures from old (older than 80 weeks) and young (6-10 weeks) mice and compared the number and size of organoids at day 5 of passage 0 and the growth rate of organoids between the two groups. RESULTS: The number and size of organoids from old mice was significantly lower than that from young mice (p < 0.0001) at day 5 of passage 0. The growth rate of old-mouse organoids from day 4 to 5 of passage 0 was significantly slower than that of young-mouse organoids (2.21 times vs. 1.16 times, p < 0.001). RNA sequencing showed that TGF-ß- and cell cycle-associated genes were associated with the aging effect. With regard to mRNA and protein levels, Smad3 and p-Smad3 in the old-mouse organoids were markedly increased compared with those in the young-mouse organoids. Decreased expression of ID1, increased expression of p16INK4a, and increased cell cycle arrest were observed in the old mouse-organoids. Treatment with SB431542, a type I TGF-ß receptor inhibitor, significantly increased the formation and growth of old-mouse organoids, and TGF-ß1 treatment markedly suppressed the formation of young-mouse organoids. In the acute dextran sulfate sodium-colitis model and its organoid experiments, the colonic epithelial regeneration after tissue injury in old mice was significantly decreased compared with young mice. CONCLUSIONS: Aging reduced the formation ability and growth rate of colonic epithelial organoids by increasing cell cycle arrest through TGF-ß-Smad3-p16INK4a signaling.

Critical parameters influencing the continuous performance of upflow microbubble airlift photocatalytic process treating pharmaceutical pollutants.

The advances in heterogeneous photocatalysts are still confined to evaluating the functional photocatalytic activity of catalysts in simple batch-mode operation. Nevertheless, the long-term stability, recovery-reusability, and cost-effectiveness of photocatalysts are critical issues in practical applications for pollution control. This study examined the critical parameters to improve the photocatalytic degradation activity of the antibiotic tetracycline and strategized successful continuous performance in a two-stage photocatalytic process adopting sequencing batch-mode microbubble upflow airlift reactor (UALR) followed by the centrifugal separation of CdS nanoparticles (NPs). The most effective strategy for NPs separation was a sequential combination of gravity separation (10 min settling) in the settling phase and subsequent high-speed centrifugation (5 min at 25,000×g) of the settled NPs sediments, providing an economic benefit by reducing the centrifugation capacity. During steady state operation under the optimal conditions, the UALR showed reliable performance, resulting in 97-91% and 85-81% degradation efficiency at 60- and 30-min reaction time per cycle, respectively. A weak basic condition (pH 8) and dissolved oxygen (DO) supplementation increased the photocatalytic activity by 12% (0.0292 min-1) and 30% (0.0363 min-1) compared to the control. Trapping studies confirmed the enhanced performance using various reactive oxygen species scavengers, revealing an increase in •OH generation (6.5%).

Lethal effects of mitochondria via microfluidics.

Tumor cells can respond to therapeutic agents by morphologic alternations including formation of tunneling nanotubes. Using tomographic microscope, which can detect the internal structure of cells, we found that mitochondria within breast tumor cells migrate to an adjacent tumor cell through a tunneling nanotube. To investigate the relationship between mitochondria and tunneling nanotubes, mitochondria were passed through a microfluidic device that mimick tunneling nanotubes. Mitochondria, through the microfluidic device, released endonuclease G (Endo G) into adjacent tumor cells, which we referred to herein as unsealed mitochondria. Although unsealed mitochondria did not induce cell death by themselves, they induced apoptosis of tumor cells in response to caspase-3. Importantly, Endo G-depleted mitochondria were ineffective as lethal agents. Moreover, unsealed mitochondria had synergistic apoptotic effects with doxorubicin in further increasing tumor cell death. Thus, we show that the mitochondria of microfluidics can provide novel strategies toward tumor cell death.

Strategic insight into enhanced photocatalytic remediation of pharmaceutical contaminants using spherical CdO nanoparticles in visible light region.

The sustainable control of pharmaceutical micropollutants in water and wastewater environments is a great challenge in the 21st century. To address these issues, unique CdO nanoparticles (NPs) were synthesized using a facile hydrothermal approach and investigated for photocatalytic control of the antibiotic tetracycline, multidrug-resistant bacteria (MDRB), and total coliform in the wastewater effluent. The NPs were characterized using a range of techniques and it exhibited a spherical-like crystal structure with a mean size of 40 nm. The vibrational stretching mode of 1419 cm-1 confirmed the formation of Cd-O (M - O). The synthesis protocol formed smoother surfaces and 1.88 eV band gap energy of CdO NPs, inducing excellent photocatalytic activity under visible LED light (blue and white) irradiation. The optimal catalyst dose and pH were 100 mg/L and 8-9, respectively. Blue light proved more effective than white light, resulting in 28% higher efficiency (93 ± 0.47%) in tetracycline degradation than white light under an identical intensity (20 mW/cm2). White light required a four-fold higher light intensity (80 mW/cm2) than blue light to induce comparable photocatalytic MDRB inactivation. Bacterial cell lysis by the photocatalytic treatment was confirmed by transmission electron microscopy (TEM). The used catalyst was easily recovered by 5 min of centrifugation and re-used without any noticeable change in the photocatalytic decomposition. The trapping experiment revealed that the CdO-based NPs contributed primarily to the generation of •O2- and •OH radicals (Type I), but the •O2- radicals were the dominant reactive oxygen species (ROS) in the photocatalytic process.

Reprogramming of cancer-associated fibroblasts by apoptotic cancer cells inhibits lung metastasis via Notch1-WISP-1 signaling.

The interplay between apoptotic cancer cells and the tumor microenvironment modulates cancer progression and metastasis. Cancer-associated fibroblasts (CAFs) play a crucial role in promoting these events through paracrine communication. Here, we demonstrate that conditioned medium (CM) from lung CAFs exposed to apoptotic cancer cells suppresses TGF-ß1-induced migration and invasion of cancer cells and CAFs. Direct exposure of CAFs to apoptotic 344SQ cells (ApoSQ) inhibited CAF migration and invasion and the expression of CAF activation markers. Enhanced secretion of Wnt-induced signaling protein 1 (WISP-1) by CAFs exposed to ApoSQ was required for these antimigratory and anti-invasive effects. Pharmacological inhibition of Notch1 activation or siRNA-mediated Notch1 silencing prevented WISP-1 production by CAFs and reversed the antimigratory and anti-invasive effects. Enhanced expression of the Notch ligand delta-like protein 1 on the surface of ultraviolet-irradiated apoptotic lung cancer cells triggered Notch1-WISP-1 signaling. Phosphatidylserine receptor brain-specific angiogenesis inhibitor 1 (BAI1)-Rac1 signaling, which facilitated efferocytosis by CAFs, participated in crosstalk with Notch1 signaling for optimal production of WISP-1. In addition, a single injection of ApoSQ enhanced WISP-1 production, suppressed the expression of CAF activation markers in isolated Thy1+ CAFs, and inhibited lung metastasis in syngeneic immunocompetent mice via Notch1 signaling. Treatment with CM from CAFs exposed to ApoSQ suppressed tumor growth and lung metastasis, whereas treatment with WISP-1-immunodepleted CM from CAFs exposed to ApoSQ reversed the antitumorigenic and antimetastatic effects. Therefore, treatment with CM from CAFs exposed to apoptotic lung cancer cells could be therapeutically applied to suppress CAF activation, thereby preventing cancer progression and metastasis.

MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes.

miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.

Preparation of P-doped CdS nanorods as an efficient photocatalyst for the degradation of the emerging pollutant tetracycline antibiotic under blue LED light irradiation.

Excessive drug usage and sewage discharges containing antibiotics have caused water contamination due to the rapid growth of pharmaceutical industries. Tetracycline (TC) is one of the most frequently applied antibiotics having a significant impact on the aquatic environment, water quality and human health and thus effective approaches for TC removal from water are urgently needed. Here, we have fabricated P-doped CdS (CdS-P0.8) nanorods (NRs) by one-step thermal phosphorization treatment for TC degradation through photocatalytic reaction in the presence of blue and white LED light irradiation. Synthesized photocatalysts were characterized to authenticate the incorporation of P atoms on the CdS NR surface using XPS, XRD, ICP-OES and EDX mapping analyses. CdS-P0.8 NRs have greater photocatalytic activity for tetracycline degradation under blue LED light irradiation. TC degradation on CdS-P0.8 NRs followed pseudo-first order kinetics for both LED light sources. In the presence of blue LED light at an intensity of 10 mW cm-2, TC degradation efficiency and pseudo-first order rate constants of CdS-P0.8 NRs for the photocatalytic degradation reaction reached 95.4% and 0.13396 min-1 in 20 minutes without any supplemental oxygen sources. Scavenging experiments demonstrate that reactive oxygen species are produced during the photocatalytic degradation of tetracycline. As a result, due to the extensive utilization of photogenerated oxidative species such as h+, O2˙- and OH˙, CdS-P0.8 NRs demonstrated high photocatalytic tetracycline degradation efficiency in 20 minutes. Our findings shed more light on nonmetal P doping on CdS materials and other semiconductors, exploring new possibilities for photocatalytic degradation to efficiently reduce the amount and toxicity of TC antibiotics in wastewater.

Antimicrobial photodynamic inactivation of wastewater microorganisms by halogenated indole derivative capped zinc oxide.

Novel 5-bromoindole (5B)-capped zinc oxide (ZnO) nanoparticles (5BZN) were synthesized to improve the antibacterial, antibiofilm, and disinfection processes for the control of microorganisms in wastewater treatment. When exposed to 5BZN, the biofilm density and cell attachment were reduced dramatically, as measured by scanning electron microscopy (SEM). The 5BZN were also investigated for photodynamic treatment of multidrug-resistant (MDR) bacteria and toxicity. The combination of 5B and ZnO exhibited strong antibacterial and antibiofilm activities against MDR bacteria even at low doses (20 µg/mL). After 12.5 mW/cm2 blue LED irradiation, the composite 5BZN showed superior photodynamic inactivation of two wastewater MDR, Enterobacter tabaci E2 and Klebsiella quasipneumoniae SC3, with cell densities reduced by 3.9 log CFU/mL and 4.7 log CFU/mL, respectively, after 120 min. The mechanism of bacterial inactivation was studied using a scavenging investigation, and H2O2 was identified mainly as the reactive species for bacterial inactivation. The 5BZN exhibited higher photodynamic inactivation towards the total coliform bacteria in wastewater effluents under a blue LED light intensity of 12.5 mW/cm2 with almost complete inactivation of the coliform bacteria cells within 40 min. Furthermore, when 5BZN (100 mg/L) was added to the reactor, the level of tetracycline antibiotic degradation was increased by 63.6% after 120 min. The toxicity test, animal model nematode studies and seed germination assays, showed that 5BZN is harmless, highlighting its tremendous potential as a self-healing agent in large-scale photodynamic disinfection processes.

Assessment of nitrogen interaction with temperature on the growth and toxin production of mat-forming toxin-producing Anagnostidinema carotinosum.

AIMS: Global warming and eutrophication contribute to the severity of cyanobacteria blooms. However, it is unclear how these factors influence the growth and toxin production of Anagnostidinema carotinosum. METHODS AND RESULTS: Based on morphological and molecular analysis, this is the first time A. carotinosum was identified in South Korea. The interactive effect of temperature (25, 30 or 34°C) and nitrogen (2.5, 3.5 or 4.5 mg NO3 -N l-1 ) on A. carotinosum growth and toxin production was studied. Increasing nitrogen limitation reflects reduced growth and chlorophyll-a content at all temperatures. However, the growth was effective under nitrogen limitation when temperatures exceeded 25°C. The maximum growth was found at 30°C, followed by 34°C under higher nitrate levels (3.5 and 4.5 mg l-1 ). In addition, the cell microcystin and anatoxin-a quota increased significantly at 25°C with increasing nitrate limitation, decreasing considerably at 30°C in the same nitrate gradient. CONCLUSION: These results suggested temperatures stimulate A. carotinosum growth at 30 and 34°C and cellular toxin quota at 25 and 34°C with increasing NO3 -N levels. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings imply that limiting nitrogen input alone can effectively reduce biomass; however, controlling A. carotinosum and its toxins at higher temperatures under nitrate limitation is necessary for water quality.

Impact of melatonin on the hydrogen peroxide treatment efficacy in Microcystis aeruginosa: Cell growth, oxidative stress response, and gene transcription.

A study was conducted to determine how melatonin (MLT), a growth regulator, affects Microcystis aeruginosa cell behaviour and how MLT exposed cells respond to hydrogen peroxide (H2O2) treatment. MLT promotes the growth, chl-a content, Fv/Fm values, and microcystins (MCs) production of M. aeruginosa at low concentrations of 1-2.5 µmol/L but suppresses the growth at high concentrations (5-10 µmol/L). The cellular and genetic responses of MLT pre-treated cells to H2O2 treatment were examined further. Further research found that the cells pre-treated with MLT were susceptible to a range of growth-promoting, inhibiting and lethal effects when exposed to higher levels of H2O2. A dose-dependent pattern was observed under conditions of 0.05-0.2 mmol/L H2O2 with 0.5-2.5 µmol/L MLT concentrations to different degrees. High doses of H2O2 (0.2 and 0.3 mmol/L) typically lead to cell lysis and release of MCs in 5.0 and 10 µmol/L MLT pre-treated cells. A decrease in SOD/CAT activities and an increase in MDA levels validated the growth reduction. Furthermore, higher cell lysis and release of intracellular MCs were observed when H2O2 was increased for 5-10 µmol/L MLT pre-treated cells. This led to a higher accumulation of extracellular MCs. The results provide insight into how MLT influences H2O2 damage and assist in identifying situations where H2O2 treatment of cyanobacterial blooms is most appropriate.

Photodynamic inactivation of multidrug-resistant bacteria in wastewater effluent using green phytochemicals as a natural photosensitizer.

The control of multidrug-resistant bacteria (MDRB) is a great challenge in the 21st century. Photodynamic treatment (PDT) is one of the promising approaches to control MDRB. In the process, powerful oxidants such as reactive oxygen species (ROS) are produced, which cause cytotoxic damage and cell death of bacteria. This study examined a new and environment-friendly strategy for the photodynamic inactivation of two MDRB (Escherichia coli and Staphylococcus aureus) and total coliform (TC) in wastewater effluent using two phytochemicals, pyrogallol (PGL) and terpinolene (TPN), along with white and blue light-emitting diode (LED) light. Fourier-transform infrared spectroscopy (FTIR) of the phytochemicals confirmed the presence of different phenolic and aromatic compounds, which can enhance the generation of ROS alongside inactivating the bacterial cells. In the PDT process, white LED light was more active in controlling MDRB than blue LED light. After 80 min irradiation with white LED light (17 mW/cm2), the MDRB bacteria were eradicated completely at a minimum inhibitory concentration (MIC) dose (0.156 mg/mL for E. coli and 0.078 mg/mL for S. aureus) of PGL. In addition, light intensity was an important parameter in photodynamic disinfection. The TC in the secondary effluent was inactivated completely by both phytochemicals after 60 min of exposure to white LED light with an intensity of 80 mW/cm2. The photosensitizing activity of phytochemicals was analyzed by a bactericidal and imidazole-RNO assay. These assays showed that PGL contributed to the generation of •OH radicals, whereas TPN produced 1O2 in the PDT process. Transmission electron microscopy (TEM) confirmed bacterial cell disruption after treatment. Overall, PDT using the phytochemicals as PS is a sustainable approach to control the MDRB and TC in wastewater successfully.

Identification and Characterization of Cancer-Associated Fibroblast Subpopulations in Lung Adenocarcinoma.

Cancer-associated fibroblasts (CAFs) reside within the tumor microenvironment, facilitating cancer progression and metastasis via direct and indirect interactions with cancer cells and other stromal cell types. CAFs are composed of heterogeneous subpopulations of activated fibroblasts, including myofibroblastic, inflammatory, and immunosuppressive CAFs. In this study, we sought to identify subpopulations of CAFs isolated from human lung adenocarcinomas and describe their transcriptomic and functional characteristics through single-cell RNA sequencing (scRNA-seq) and subsequent bioinformatics analyses. Cell trajectory analysis of combined total and THY1 + CAFs revealed two branching points with five distinct branches. Based on Gene Ontology analysis, we denoted Branch 1 as "immunosuppressive", Branch 2 as "neoantigen presenting", Branch 4 as "myofibroblastic", and Branch 5 as "proliferative" CAFs. We selected representative branch-specific markers and measured their expression levels in total and THY1 + CAFs. We also investigated the effects of these markers on CAF activity under coculture with lung cancer cells. This study describes novel subpopulations of CAFs in lung adenocarcinoma, highlighting their potential value as therapeutic targets.

Layered KTO/BiOCl nanostructures for the efficient visible light photocatalytic degradation of harmful dyes.

Novel KTO/BiOCl nanostructured photocatalysts with various weight proportions were synthesized using a simple hydrothermal process. The as-prepared nanostructured composite catalysts were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, UV-vis diffused reflectance spectroscopy, Raman spectroscopy, field emission scanning electron microscopy, transmission electron microscopy with high resolution, X-ray photoelectron spectroscopy, and photoluminescence (PL). The photocatalytic activity of prepared catalysts was examined using Rhodamine B (RhB) and Congo Red (CR) as the aimed pollutants. BiOCl nanoparticles were distributed uniformly on the surface of the K2Ti4O9 nanobelts. The optical properties showed that the layered titanate with BiOCl nanostructured photocatalyst displayed improved photoresponsivity due to the narrowed bandgap. The PL results showed that the greater inhibition of the electron-hole recombination process and KTO/BiOCl with a mass proportion of 20% revealed the most favorable photocatalytic behavior. The rate constant of RhB and CR degradation was five times as high as that of the bare BiOCl and titanate. The superior photocatalytic performance was attributed to the advancement of heterojunction between the KTO nanobelt and BiOCl. The KTO/BiOCl nanostructure is a promising visible, active photocatalyst, and the photocatalytic mechanism is discussed using the possible band structures of BiOCl and KTO.

Plasma Membrane Localization of CD36 Requires Vimentin Phosphorylation; A Mechanism by Which Macrophage Vimentin Promotes Atherosclerosis.

Vimentin is a type III intermediate filament protein expressed in cells of mesenchymal origin. Vimentin has been thought to function mainly as a structural protein and roles of vimentin in other cellular processes have not been extensively studied. Our current study aims to reveal functions of vimentin in macrophage foam cell formation, the critical stage of atherosclerosis. We demonstrated that vimentin null (Vim -/ - ) mouse peritoneal macrophages take up less oxidized LDL (oxLDL) than vimentin wild type (Vim +/+) macrophages. Despite less uptake of oxLDL in Vim -/ - macrophages, Vim +/+ and Vim -/ - macrophages did not show difference in expression of CD36 known to mediate oxLDL uptake. However, CD36 localized in plasma membrane was 50% less in Vim -/ - macrophages than in Vim +/+ macrophages. OxLDL/CD36 interaction induced protein kinase A (PKA)-mediated vimentin (Ser72) phosphorylation. Cd36 -/ - macrophages did not exhibit vimentin phosphorylation (Ser72) in response to oxLDL. Experiments using phospho-mimetic mutation of vimentin revealed that macrophages with aspartate-substituted vimentin (V72D) showed more oxLDL uptake and membrane CD36. LDL receptor null (Ldlr -/ - ) mice reconstituted with Vim -/ - bone marrow fed a western diet for 15 weeks showed 43% less atherosclerotic lesion formation than Ldlr -/ - mice with Vim +/+ bone marrow. In addition, Apoe -/ -Vim- / - (double null) mice fed a western diet for 15 weeks also showed 57% less atherosclerotic lesion formation than Apoe -/ - and Vim +/+mice. We concluded that oxLDL via CD36 induces PKA-mediated phosphorylation of vimentin (Ser72) and phosphorylated vimentin (Ser72) directs CD36 trafficking to plasma membrane in macrophages. This study reveals a function of vimentin in CD36 trafficking and macrophage foam cell formation and may guide to establish a new strategy for the treatment of atherosclerosis.

Fabrication and application of novel high strength sulfonated PVDF ultrafiltration membrane for production of reclamation water.

Advanced treated water (ATW) produced in wastewater treatment facilities was assessed as an excellent alternative water resource that can be used as reclamation water, such as indirect and direct potable reuse. The development of cutting-edge technology for simple but best practices is essential for the reliable production of safe reclamation water from wastewater. This study prepared a novel high strength sulfonated polyvinylidene fluoride (HSPVDF) ultrafiltration membrane and investigated to produce ATW, and performances were compared to sulfonated PVDF (SPVDF) (which was prepared without thermal treatment) and bare PVDF. To compare the properties of HSPVDF to hydrocarbon polymer, the polyetherimide (PEI) and Sulfonated PEI (SPEI) membrane were prepared. HSPVDF showed excellent membrane morphology, porosity, MWCO, and hydrophilicity, resulting in higher pure water flux (712 ± 6 L m-2 h-1) antifouling properties (Rir 1.3% and FRR 98.6%) compared to PVDF. It is an interesting fact that the tensile strength of the HSPVDF (3.4 ± 0.2 MPa) tremendously increased (3 folders) when compere to PVDF (1.3 ± 0.1 MPa). The HSPVDF membrane showed good removal efficiency up to 96 ± 05% and 97 ± 09% rejection for bovine serum albumin (BSA) and humic acid (HA), respectively. The membrane application studies for wastewater treatment showed that the tertiary HSPVDF UF membrane filtration following the nutrient removal activated sludge (NRAS) process can produce reliable and economic performance (125 ± 2 L m-2 h-1, 0.25 ± 0.05 NTU, no pathogens), suggesting that it can be a best practice technique that can replace the complicated multi-staged tertiary processes to produce reclamation water.